Caspase 8 anticorps

N° du produit ABIN6655461

N° du produit (Fournisseur): 200-301-h64

Cet anticorps Souris Monoclonal détecte spécifiquement Caspase 8 dans WB, IHC et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour Caspase 8 anticorps (ABIN6655461)

Antigène: Caspase 8 (CASP8)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 90A992

Détail du produit anti-Caspase 8 anticorps

N° du produit (Fournisseur): 200-301-h64

Fournisseur: Rockland

Fonction: Caspase-8 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-8 from Chimpanzee, Human, Rhesus Monkey and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-Caspase-8 Antibody was purified by Protein G chromatography.

Immunogène: Caspase-8 Antibody was produced in mice prepared by repeated immunizations with amino acids at the internal portion of human Caspase-8.

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 5 μg/mL

Flow_Cytometry_Dilution: 0.1-0.5 μg/mL

Western_Blot_Dilution: 0.5-2 μg/mL

Commentaires:

Anti-Caspase-8 antibody is tested for use by WB, Flow, and IHC-P. Expect a band approximately 55 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Caspase 8

Antigène: Caspase 8 (CASP8)

Autre désignation: Caspase-8

Sujet:

Synonyms: MCH5Caspase-8, CASP-8, CASP8, 3.4.22.61, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, Caspase-8 subunit p18, Caspase-8 subunit p10

Background: Anti-Caspase-8 detects human Caspase. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Anti-Caspase-8 antibody is ideal for investigators involved in apoptosis research.

Gene Name: Casp8

ID gène: 841

NCBI Accession: NP_001073593

UniProt: Q14790

Pathways: Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades